Journal: American Journal of Cancer Research
Article Title: Upregulation of Cyclin B2 ( CCNB2 ) in breast cancer contributes to the development of lymphovascular invasion
doi:
Figure Lengend Snippet: Representative photomicrographs of tumour cell adhesion across vascular and lymphatic endothelial cells (HUVECs, DLECs) (A) MCF-7, (B) ZR-75-1 cells and (C) SK-BR-3. (A-C) Silencing CCNB2 decreased the number of all BC cell lines adhered with HUVECs and DLECs. Representative photomicrographs of tumour cell (MCF-7, ZR-75-1 and SK-BR-3) transmigration across (D) HUVECs and (E) DLECs. (D, E) The number of tumour cells transmigrated across HUVECs and DLECs was higher in the control group compared to the KD group. Results shown are mean ± standard error of the mean (SEM) of three independent experiments. The P-values *<0.05, **<0.01, ***<0.001 and ****<0.0001.
Article Snippet: Primary human umbilical vein endothelial cells (HUVECs) and dermal lymphatic endothelial cells (DLECs) were purchased from Promocell (Heidelberg, Germany).
Techniques: Transmigration Assay